This landmark Off-Broadway theatre was opened in 1996 by the Tony Award winning producer Daryl Roth and comprises three exceptional venues. The Daryl Roth Theater is currently hosting the musical Titanique. The story begins when Céline Dion hijacks a Titanic Museum tour and enchants the audience with her totally wild take, recharting the course of Titanic’s beloved moments and characters with her iconic song catalog.

The unique main theatre space has hosted such non-traditional hits as the long running De La Guarda and Fuerza Bruta, which played a combined 14 years; Almost, Maine; In & Of Itself; Gloria: A Life; Hannah Gadbsy’s Douglas; and Peter Dinklage in Cyrano: The Musical. The intimate DR2 has been home to shows including the U.S. premiere of Thom Pain (based on nothing), Stars of David, and That Golden Girls Show, as well as the DR2 Kids programing that introduces the youngest patrons to theatre; like Dear Edwina and The Very Hungry Caterpillar. The D-Lounge is the versatile cabaret space.

The Daryl Roth Theatre is an off-Broadway performance space at 101 East 15th Street, at the northeast corner of the intersection with Union Square East, near Union Square, Manhattan, New York City. The theater, opened in 1998, is housed in the four-story Union Square Savings Bank building, which was designed by Henry Bacon and built in 1905–1907. The original structure, a New York City landmark, houses a theater that can accommodate 300 seated or 499 standing patrons. The DR2 Theatre, located in an annex at 103 East 15th Street, seats 99

In 1993, the House of Blues concert-venue company bought the bank and its annex at 103 East 15th Street for a combined $2.06 million. Isaac Tigrett, the House of Blues' founder, planned to open the venue with a live-music venue and 300-seat eatery in 1994. This drew concern among preservationists, who wanted the bank to be preserved in its original state, though Tigrett stated that he would not change the inside of the bank. The project was delayed, and by March 1995, construction was reported to be imminent. However, that June, the House of Blues withdrew its application for a liquor license, which would have enabled the restaurant to contain a bar. Manhattan Community Board 5, which covers Union Square, expressed concerns about the effects of a concert venue in the neighborhood, so the House of Blues agreed to delay construction in exchange for postponement of a public hearing.

In 1996, the building was acquired by Daryl Roth, who had devised plans to turn the bank into an off-Broadway venue. Upon the theater's opening in 1998, The New York Times described the bank building as one of a "growing number of unconventional spaces" that were converted into theaters. The DR2 Theatre was opened in 2002 within the former banking annex in 103 East 15th Street.

While Fuerza Bruta was in production at the theater from 2005 to 2016, the theater entrance was through a side door on the 15th Street side, while the main building entrance at Union Square was unused. In 2017, while making his theatrical directorial debut with In and Of Itself, Frank Oz changed the layout of the theater to incorporate its visually striking Union Square facade as an entrance to the theater. The renovations required moving the box office around, but upon the completion of work, the audience was able to enter through the front doors on Union Square East.

At the DR2 theater, Bunnicula ran between February and April 2013, while That Golden Girls Show ran from October 2016 to January 2017.